This tool automatically estimates the Limit of Blank (LoB) and the Limit of Detection (LoD) for a target gene complying with EP17-A2 standards.
The values are computed given a set of negative controls (at least 30 replicated are recommended), a set of low-level samples (at least 5 different samples with a minimum of 6 replicates each are recommended), and a confidence level. To use the calculator only to calculate the LoB, no values are required for the low-level samples column.
To learn more about LOD and LOB for digital PCR, check out this Technical Note available here: How to characterize the Limit of Blank and the Limit of Detection in Crystal Digital PCR™
Below is an example of common usage. Download it and follow the steps to get your results.
How to use the tool?
- Get the template
- Complete the file with your information
- Post the completed file and click Submit to automatically download results
1 -Download the template
Get an example
2 - Select data